For research use only. NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE.
Catalog Number: MPE0002926
Product Quantity: 5mg/20mg
Price: Varies
Availability: In Stock
The FLAG Epitope Peptide consists of eight amino acids (DYKDDDDK) and represents a versatile fusion tag for the purification of recombinant proteins. It can be enzymatically removed with enterokinase which cleaves C-terminally to the tag sequence. The peptide is commonly used for the elution of immunoaffinity chromatography-purified recombinant proteins.
Product Name: | FLAG Epitope Peptide, DYKDDDDK |
Molecular Formula: | C41H60N10O20 |
Molecular Weight: | 1012.98 |
Sequence: | DYKDDDDK |
Three letter code: | H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH Trifluoroacetate salt |
Length (aa): | 8 |
Peptide Purity (HPLC): | >95%; 98% and 99% purity available upon request |
Quantity/Unit: | 1 Vial |
* Optional Service: | TFA Removal Service is available upon request. |
Source: | Synthetic |
Storage Guidelines: | Store at -20°C for up to 1 year. should be refrigerated after reconstitution. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides |
Solubility: | Soluble in water |
Appearance: | White to off-white powder |
Shipping: | Peptides are shipped at ambient temperature by standard shipment process. |
About TFA salt: | Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA. TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others. It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR. TFA Removal Service is recommended for: > Peptides that will be used in cellular assays > Peptides that will be used as APIs or in manufactured products > For hydrophilic peptides containing numerous basic residues |
Custom Peptide Synthesis: FLAG Epitope Peptide, DYKDDDDK peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression. Read more...
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