Cytolysin peptide
For research use only. NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE.
Catalog Number: MPE0005773
Product Quantity: 5mg/20mg
Price: Varies
Availability: In Stock
Product Information
Enterococcus faecalis senses target cells and in response expresses cytolysin. The cytolysin consists of two structural subunits, CylLL and CylLS, that are activated by an extracellular protease, CylA. In the absence of target cells, the other subunit (CylL(L)'') forms a complex with CylL(S)'', blocking it from autoinducing the operon. When target cells are present, however, CylL(L)'' binds preferentially to the target, allowing free CylL(S)'' to accumulate above the induction threshold to express high levels of cytolysin (Coburn PS et al., 2004). The two chains work synergistically at a ratio of 1:1. CylLS contains two thioether bonds: T1-C5 and S17-C20. In addition, T2 and T7 are dehydrated. The AMINO ACID sequence of CylLL is TTPVCAVAATAAASSAACGWVGGGIFTGVTVWSLKHC. There are three thioether bonds: T1-C5, S14-C18, S34-C38. In addition, T2, T10, S15, and T30 are dehydrated. The peptide may also contain usual lanthionine stereochemistry (Tang and van der Donk, 2013 Nature Chem Biol ). Updated 1/2013; 9/2014. Cytolysin has Antimicrobial activity. The source of Cytolysin is Enterococcus faecalis.
| Product Name: | Cytolysin peptide |
| Sequence: | TTPACFTIGLGVGALFSAKFC |
| Three letter code: | Thr-Thr-Pro-Ala-Cys-Phe-Thr-Ile-Gly-Leu-Gly-Val-Gly-Ala-Leu-Phe-Ser-Ala-Lys-Phe-Cys |
| Length (aa): | 21 |
| Peptide Purity (HPLC): | >95%; 98% and 99% purity available upon request |
| Quantity/Unit: | 1 Vial |
| * Optional Service: | TFA Removal Service is available upon request. |
Technical Information
| Source: | Synthetic |
| Storage Guidelines: | Store at -20°C for up to 1 year. should be refrigerated after reconstitution. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides |
| Solubility: | Soluble in water |
| Appearance: | White to off-white powder |
| Shipping: | Peptides are shipped at ambient temperature by standard shipment process. |
| About TFA salt: | Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA. TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others. It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR. TFA Removal Service is recommended for: > Peptides that will be used in cellular assays > Peptides that will be used as APIs or in manufactured products > For hydrophilic peptides containing numerous basic residues |
Related Products / Services
Custom Peptide Synthesis: Cytolysin peptide peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression. Read more...
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