For research use only. NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE.
Catalog Number: MPE0002512
Product Quantity: 5mg/20mg
Price: Varies
Availability: In Stock
CF-14(RIVELTLPRVSVRL-NH2) is a novel antibacterial peptide, derived from the epidermal mucus of catfish. CF-14 showed antibacterial activity against S. putrefaciens and S. aureus, as well as Escherichia coli compared to negative controls. When the concentration of CF-14 was 62.5 μg/mL, the antibacterial effect of CF-14 against S. putrefaciens was similar to that of 50 μg/mL of ampicillin. CF-14 displays potent activity against gram-negative (S. putrefaciens) bacteria and gram-positive bacteria (S. aureus). Further, the MIC of CF-14 against S. putrefaciens was 62.5 μg/mL. The antimicrobial activity of CF-14 showed no obvious change after heat treatment for 30 min at either at 40 °C, 60 °C, or 80 °C, and slight inactivation after treatment at 100 °C for 30 min. CF-14 remained stable after 24 h after exposure to a pH values ranging from 4.0 to 12.0, but the antimicrobial activity of CF-14 decreased after exposure to an acidic environment (pH < 4). Finally, CF-14 was susceptible to proteases as no antimicrobial activity was observed after treatment with trypsin, pepsin, or proteinase K. CF-14 has the ability to enhance the membrane permeability of S. putrefaciens. Moreover, observations performed using scanning electron and confocal microscopy indicated that CF-14 could penetrate into the cell membranes of S. putrefaciens and accumulate in bacterial cells, but did not break down cell membranes. Further, electrophoresis analysis demonstrated that CF-14 possesses DNA-binding affinity.
Product Name: | CF-14 peptide |
Molecular Formula: | C74H136N24O18 |
Molecular Weight: | 1650.01 |
Sequence: | H-RIVELTLPRVSVRL-Amidation |
Three letter code: | H-Arg-Ile-Val-Glu-Leu-Thr-Leu-Pro-Arg-Val-Ser-Val-Arg-Leu-Amidation |
Length (aa): | 26 |
Peptide Purity (HPLC): | >95%; 98% and 99% purity available upon request |
Quantity/Unit: | 1 Vial |
* Optional Service: | TFA Removal Service is available upon request. |
Source: | Synthetic |
Storage Guidelines: | Store at -20°C for up to 1 year. should be refrigerated after reconstitution. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides |
Solubility: | Soluble in water |
Appearance: | White to off-white powder |
Shipping: | Peptides are shipped at ambient temperature by standard shipment process. |
About TFA salt: | Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA. TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others. It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR. TFA Removal Service is recommended for: > Peptides that will be used in cellular assays > Peptides that will be used as APIs or in manufactured products > For hydrophilic peptides containing numerous basic residues |
Custom Peptide Synthesis: CF-14 peptide peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression. Read more...
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